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Image Search Results


Na V 1.2 labels Domain 3 in majority of afferents, first nodes, and stromal paranodes. (A, A’) Na V 1.2 ( green ) labels the calyx outer surfaces (Domain 3, arrows ) of D afferents in the extrastriolar zone of the utricular macula from an adult female rat. The calyx inner surfaces are marked with Caspr1 ( Caspr, red in A). The beginning of two parent axons (arrowheads) can be seen in the neuroepithelium, but could not be traced into the stroma, where the heminodes of most D units are located, presumably because they go out of the plane of this single section. Inset in A’, upper right , schematic of entire calyx, showing Domain 3 in red. (B) Na V 1.2 antibody ( green ) labels mostly stromal nodes ( arrowheads ), but not heminodes ( arrows ), both of which are marked with an ezrin antibody ( red ). (C) Deeper in the stroma of the crista in a maximum intensity projection, many nodes can be observed. The Na V 1.2 ( green ) extends beyond the boundaries of the node, marked by ezrin ( red ). C insets . Na V 1.6 antibody (in this case red ) immunostains two nodes of Ranvier in the stroma of a rat saccular macula. Similar to (C), Na V 1.2 ( green ) also stains the nodes (seen after removal of the Na V 1.6 immunolabel ( red ) in the bottom half of each inset) and extends well beyond each node into the paranode. Scale bars: A–C = 10 µm; C inset = 5 µm.

Journal: The Journal of Comparative Neurology

Article Title: Distribution of Voltage‐Gated Sodium Channels and Scaffolding Proteins on Vestibular Calyx Ending Delineates the Axon Initial Segment

doi: 10.1002/cne.70127

Figure Lengend Snippet: Na V 1.2 labels Domain 3 in majority of afferents, first nodes, and stromal paranodes. (A, A’) Na V 1.2 ( green ) labels the calyx outer surfaces (Domain 3, arrows ) of D afferents in the extrastriolar zone of the utricular macula from an adult female rat. The calyx inner surfaces are marked with Caspr1 ( Caspr, red in A). The beginning of two parent axons (arrowheads) can be seen in the neuroepithelium, but could not be traced into the stroma, where the heminodes of most D units are located, presumably because they go out of the plane of this single section. Inset in A’, upper right , schematic of entire calyx, showing Domain 3 in red. (B) Na V 1.2 antibody ( green ) labels mostly stromal nodes ( arrowheads ), but not heminodes ( arrows ), both of which are marked with an ezrin antibody ( red ). (C) Deeper in the stroma of the crista in a maximum intensity projection, many nodes can be observed. The Na V 1.2 ( green ) extends beyond the boundaries of the node, marked by ezrin ( red ). C insets . Na V 1.6 antibody (in this case red ) immunostains two nodes of Ranvier in the stroma of a rat saccular macula. Similar to (C), Na V 1.2 ( green ) also stains the nodes (seen after removal of the Na V 1.6 immunolabel ( red ) in the bottom half of each inset) and extends well beyond each node into the paranode. Scale bars: A–C = 10 µm; C inset = 5 µm.

Article Snippet: Na V 1.2 (ms) , NeuroMab , 75‐024 RRID:AB_2184030 , , X (Br) , , X , , Datasheet.

Techniques: Immunolabeling